Virginie Bottero

Virginie Bottero

  • Assistant Professor, Biology
    Email Address:
    Office location:
    Straz Center 161

    After completing her doctorate in molecular and cellular biology in 2002 from the University of Nice Sophia-Antipolis, France, Professor Virginie Bottero joined the Salk Institute for Biological Studies to perform a post-doctoral fellowship. During her Ph.D. and postdoc, she became an expert in cell signaling. At Rosalind Franklin University of Medicine and sciences (RFUMS), North Chicago Illinois, Prof. Bottero’s research primarily focused on the signaling pathways activated by the human herpesvirus 8 (KSHV). In the past years, she has participated in different projects in the neuroscience field. Recently, her team applied bioinformatic and network analysis to uncover Parkinson’s disease biomarkers. Prof. Bottero also applied different bioinformatic tools such as meta-analysis and network analysis to better understand gene regulation in different dementia.

    During the last 10 years, she was fortunate to have the opportunity to participate in teaching at the graduate and undergraduate levels. At RFUMS, she taught lectures in cancer biology, molecular and cellular biology, and advanced virology. Outside of her teaching responsibilities Prof. Bottero also participated in other faculty functions at the school including symposium poster judging and interviewing medical student candidates. At Lake Forest College, she taught entry-level, nonbiologist students (“microbes, health and society” and “genes, cells, and disease”) as well as seniors biology majors (“molecular neuroscience” and “microbiology”). Both courses included lectures as well as a laboratory component. Prof. Bottero also participated in the laboratory portion of the course “Molecules, Genes, and Cells.” Finally, she is a preceptor for the Rosalind Franklin University Medical School where she guides some small groups of first-year and second-year medical students through some clinical cases.

    • Post-doctoral Fellowship, Rosalind Franklin University Medical School, North Chicago
    • Post-doctoral Fellowship, The Salk Institute for Biological Research, La Jolla
    • PhD Nice Sophia-Antipolis University, France
    • Engineer in Pharmacology Polytechnic School of Nice Sophia-Antipolis, France
    • MS Nice Sophia-Antipolis University, France
    • BS Nice Sophia-Antipolis University, France
    • BIO2010 Anatomy and Physiology for the Health Professional I
    • BIO2020 Anatomy and Physiology for the Health Professional II

    “After working for several years in gene regulation applied to cancer biology and virology, I study mRNA expression as biomarkers for neurodegenerative disorders.”

    • NIH R21 Grant (AI097540) (2012-2014).
    • INSERM postdoctoral fellowship (2003-2004).
    • La Ligue contre le Cancer postdoctoral fellowship (2002-2003).
    • Association of Research Against Cancer doctoral prolongation fellowship (2001-2002).
    • Governmental Doctoral grant (1998-2001).

    Bottero V, Santiago JA, Quinn JP, and Potashkin JA. Key Disease Mechanisms Linked to Amyotrophic Lateral Sclerosis in Spinal Cord Motor Neurons. Front Mol Neurosci. 2022 Mar 15;15:825031. doi: 10.3389/fnmol.2022.825031. eCollection 2022. PMID: 35370543

    Bottero V, Alrafati F, Santiago JA and Potashkin JA. Transcriptomic and Network Meta-Analysis of Frontotemporal Dementias. Front Mol Neurosci. 2021. Oct 15;14:747798. doi: 10.3389/fnmol.2021.747798. eCollection 2021. PMID: 34720873

    Bottero V, Powers D, Yalamanchi A, Quinn JP, Potashkin JA. Key Disease Mechanisms Linked to Alzheimer’s Disease in the Entorhinal Cortex. Int J Mol Sci. 2021 Apr 10;22(8):3915. PMID: 33920138

    Bottero V, Potashkin JA. A Comparison of Gene Expression Changes in the Blood of Individuals Consuming Diets Supplemented with Olives, Nuts or Long-Chain Omega-3 Fatty Acids. Nutrients. 2020 Dec 8;12(12):3765. PMID: 33302351.

    Potashkin JA, Bottero V, Santiago JA, Quinn JP. Bioinformatic Analysis Reveals Phosphodiesterase 4D-Interacting Protein as a Key Frontal Cortex Dementia Switch Gene. Int J Mol Sci. 2020 May 27;21(11):3787. PMID: 32471155 

    Santiago JA, Bottero V, Potashkin JA. Transcriptomic and Network Analysis Identifies Shared and Unique Pathways across Dementia Spectrum Disorders. Int J Mol Sci. 2020 Mar 17;21(6):2050. PMID: 32192109.

    Schrank S, McDaid J, Briggs CA, Mustaly-Kalimi S, Brinks D, Houcek A, Singer O, Bottero V, Marr RA, Stutzmann GE. Human-Induced Neurons from Presenilin 1 Mutant Patients Model Aspects of Alzheimer’s Disease Pathology. Int J Mol Sci. 2020 Feb 4;21(3):1030. PMID: 32033164.

    Santiago JA, Bottero V, Potashkin JA. Transcriptomic and Network Analysis Highlight the Association of Diabetes at Different Stages of Alzheimer’s Disease. Front Neurosci 2019, 13, 1273. PMID: 31849586.

    Bottero V, Potashkin JA. Meta-Analysis of Gene Expression Changes in the Blood of Patients With Mild Cognitive Impairment and Alzheimer’s Disease Dementia. Int J Mol Sci 2019, 20(21). PMID: 31671574

    Potashkin JA, Bottero V, Santiago JA, Quinn JQ. Computational Identification of Key Genes That May Regulate Gene Expression Reprogramming in Alzheimer’s Patients PLoS One 2019, 14 (9), e0222921. PMID: 31545826.

    Bottero V, Santiago JA, and Potashkin JA. PTPRC is a potential biomarker for Parkinson and Progressive Supranuclear Palsy Disorders. Journal of Parkinson’s Disease. 2018, 8 (4), 529- 537 PMID: 30248063

    Santiago JA, Bottero V, and Potashkin JA. Evaluation of RNA Blood Biomarkers in the Parkinson’s Disease Biomarkers Program Front Aging Neurosci 2018. May 29;10:157. PMID: 29896099

    Santiago JA, Bottero V, Potashkin JA. Evaluation of RNA Blood Biomarkers in the Parkinson’s Disease Biomarkers Program. Front Aging Neurosci. 2018 May 29;10:157. doi: 10.3389/fnagi.2018.00157. PMID: 29896099

    Mohanan Valiya Veettil, Binod Kumar, Mairaj Ansari, Dipanjan Dutta, Jawed Iqbal, Olsi Gjyshi, Virginie Bottero, and Bala Chandran. ESCRT-0 Component Hrs Promotes Macropinocytosis of Kaposi’s Sarcoma-Associated Herpesvirus in Human Dermal Microvascular Endothelial Cells. J Virol. 2016 Mar 28;90(8):3860-72. PMID: 26819309

    Stephanie Flaherty, Olsi Gjyshi, Mohanan Valiya Veettil, Karen E. Johnson, Bala Chandran and Virginie Bottero*. Kaposi’s Sarcoma-Associated Herpesvirus Induces Nrf2 Activation in Latently-Infected Endothelial Cells through SQSTM1 Phosphorylation and Interaction with Polyubiquitinated Keap1. J Virol. 2015 Feb 15;89(4):2268-86. PMID: 25505069. * corresponding author

    Karen E. Johnson, Virginie Bottero, Vivek Vikram Singh, Stephanie Flaherty, Bala Chandran Host Nuclear Innate Immune Sensor, IFI16, Restricts HSV-1 Replication by Regulating Gene Expression of all Temporal Classes of HSV-1 genes and Inducing Chromatin Remodeling. PLoS Pathog. 2014 Nov 6;10(11):e1004503. PMID: 25375629.

    Olsi Gjyshi, Virginie Bottero, Mohanan Valliya Veettil, Sujoy Dutta, Vivek Vikram Singh, Leela Chikoti and Bala Chandran Kaposi’s Sarcoma-Associated Herpesvirus Induces Nrf2 6 During De Novo Infection of Endothelial Cells to Create a Microenvironment Conducive for Infection and Establishment of Latency. PLoS Pathog. 2014 Oct 23;10(10):e1004460. PMID: 25340789.

    Mohanan Valliya Veettil, Dipanjan Dutta, Virginie Bottero, Chirosree Bandyopadhyay, Olsi Gjyshi, Neelam Sharma-walia, Sujoy Dutta and Bala Chandran. Glutamate secretion and metabotropic glutamate receptor 1 expression during Kaposi’s sarcoma-associated herpesvirus infection promotes cell proliferation. PLoS Pathog. 2014 Oct 9;10(10):e1004389. PMID: 25299066.

    Sujoy Dutta, Chirosree Bandyopadhyay, Virginie Bottero, Mohanan V. Veettil, Lydia Wilson, Michael R. Pins, Karen E. Johnson, Case Warshall and Bala Chandran. Angiogenin interacts with the plasminogen activation system at the cell surface of breast cancer cells to regulate plasmin formation and cell migration. Molecular Oncology Journal. 2014 May;8(3):483-507. PMID: 24457100.

    Virginie Bottero*, Sathish Sadagopan, Karen E. Johnson, Sujoy Dutta, Mohanan Valiya Veettil and Bala Chandran. Kaposi’s Sarcoma-Associated Herpesvirus Positive Primary Effusion Lymphoma Tumor Formation in NOD/SCID Mice Is Inhibited by Neomycin and Neamine Blocking Angiogenin’s Nuclear Translocation. J Virol. 2013 Nov;87(21):11806-11820. PMID: 23986578. * corresponding author

    Singh VV#, Kerur N#, Bottero V#, Dutta S, Chakraborty S, Ansari MA, Paudel N, Chikoti L, Chandran B. Kaposi’s Sarcoma-Associated Herpesvirus Latency in Endothelial and B cells Activates Interferon Gamma-Inducible Protein 16 (IFI16) Mediated Inflammasomes. J Virol. 2013 Apr;87(8):4417-31. PMID: 23388709. # Authors contributed equally.

    Bottero V *, Chakraborty S, Chandran B. Reactive Oxygen Species Are Induced by Kaposi’s Sarcoma-Associated Herpesvirus Early during Primary Infection of Endothelial Cells To Promote Virus Entry. J Virol. 2013 Feb;87(3):1733-49. PMID: 23175375. * corresponding author

    Chakraborty S, ValiyaVeettil M, Bottero V, Chandran B. Kaposi’s sarcoma-associated herpesvirus interacts with EphrinA2 receptor to amplify signaling essential for productive infection. Proc. Natl. Acad. Sci. USA. 2012 May 8;109(19):E1163-72. PMID: 22509030.

    Sharma-Walia, N, Patel. K, Chandran K, Bottero V, Kerur N, Paul AG, and Chandran B. Role of v FLIP in COX-2 induction and KSHV latency. Oncogenesis. (2012) 1, e5; doi:10.1038/oncsis.2012.5. PMID: 23552603.

    Sadagopan S, Veettil MV, Chakraborty S, Sharma-Walia N, Paudel N, Bottero V, Chandran B. Angiogenin functionally interacts with p53 and regulates p53-mediated apoptosis and cell survival. Oncogene. 2012 Jan 23. doi:10.1038/onc.2011.648. PMID: 22266868.

    Kerur N, Veettil MV, Sharma-Walia N, Bottero V, Sadagopan S, Otageri P, Chandran B. IFI16 acts as a nuclear pathogen sensor to induce the inflammasome in response to Kaposi Sarcoma-associated herpesvirus. Cell Host Microbe. 2011 May 19;9(5):363-75. PMID: 7 21575908.

    Sadagopan S, Veettil MV, Paudel N, Bottero V, Chandran B. Kaposi`s sarcoma-associated herpesvirus (KSHV) induced angiogenin play roles in latency via PLCg pathway and blocking angiogenin inhibits lantent gene expression and induces lytic cycle. J Virol. 2011 Mar;85(6):2666-85. PMID: 21209106.

    Bottero V*, Patel K, Sharma-Walia N, Sadagopan S, Chandran B. TGFb Activating Kinase 1 (Tak1) is involved in NF-kB activation and tumorigenesis induced by Kaposi Sarcoma- associated herpes virus (KSHV) G protein-coupled receptor (vGPCR). J Virol. 2011 Mar;85(5):1980-93. PMID: 21159881. * corresponding author

    Kerur N, Veettil MV, Sharma-Walia N, Sadagopan S, Bottero V, Paul AG, Chandran B. Characterization of entry and infection of monocytic THP-1 cells by Kaposi’s sarcoma associated herpesvirus (KSHV): role of heparan sulfate, DC-SIGN, integrins and signaling. Virology. 2010 Oct 10;406(1):103-16. PMID: 20674951.

    Sharma-Walia N, Bottero V#, Paul AG#, Sadagopan S, Veettil MV, Kerur N, Chandran B. Kaposi’s sarcoma associated herpes virus (KSHV) induced cyclooxygenase (COX)-2: A key factor in latency, inflammation, angiogenesis, cell survival and invasion. PLoS Pathogens. 2010 Feb 12;6(2):e1000777. PMID: 20169190. # Both authors contributed equally.

    Bottero V, Sharma-Walia N, Kerur N, Paul AG, Sadagopan S, Cannon M, Chandran B. Kaposi Sarcoma-associated herpes virus (KSHV) G protein-coupled receptor (vGPCR) activates the ORF50 lytic switch promoter: A potential positive feedback loop for sustained ORF50 gene expression. Virology. 2009;392(1):34-51. PMID: 19640558.

    Xia Y, Padre RC, De Mendoza TH, Bottero V, Tergaonkar VB, Verma IM. Phosphorylation of p53 by IkappaB kinase 2 promotes its degradation by beta-TrCP. Proc Natl Acad Sci U S A. 2009;106(8):2629-34. PMID: 19196987.

    Sadagopan S, Sharma-Walia N, Veettil MV, Bottero V, Levine R, Vart RJ, Chandran B. Kaposi’s Sarcoma Associated Herpes Virus (KSHV/HHV-8) Upregulates Angiogenin During Infection of Human Dermal Microvascular Endothelial Cells Which Induces 45SrRNA Synthesis, Anti-apoptosis, Cell Proliferation, Migration and Angiogenesis. J Virol. 2009;83(7):3342-64. PMID: 19158252.

    Sivakumar R, Sharma-Walia N, Raghu H, Veettil MV, Sadagopan S, Bottero V, Varga L, Levine R, Chandran B. Kaposi’s sarcoma-associated herpesvirus induces sustained levels of vascular endothelial growth factors A and C early during in vitro infection of human microvascular dermal endothelial cells: biological implications. J Virol. 2008;82(4):1759-76. PMID: 18057235.

    Frelin C, Imbert V, Bottero V, Gonthier N, Samraj AK, Schulze-Osthoff K, Auberger P, Courtois G, Peyron JF. Inhibition of the NF-kB survival pathway via caspase-dependent cleavage of the IKK complex scaffold protein and NF-kB essential modulator NEMO. Cell Death Differ . 2008;15(1):152-60. PMID: 17932497.

    Raghu H, Sharma-Walia N, Veettil MV, Sadagopan S, Caballero A, Sivakumar R, Varga L, Bottero V, Chandran B. Lipid Rafts of Primary Endothelial Cells Are Essential for Kaposi’s Sarcoma-Associated Herpesvirus (KSHV/HHV-8) Induced PI3-K and RhoA-GTPases Critical for Microtubule Dynamics and Nuclear Delivery of Viral DNA but Dispensable for Binding and Entry. J Virol. 2007;81(15):7941-59. PMID: 17507466.

    Sadagopan S, Sharma-Walia N, Veettil MV, Raghu H, Sivakumar R, Bottero V, Chandran B. Kaposi’s sarcoma-associated herpesvirus induces sustained NF-kappaB activation during de novo infection of primary human dermal microvascular endothelial cells that is essential for viral gene expression. J Virol. 2007;81(8):3949-68. PMID: 17287275.

    Magne N, Toillon RA, Bottero V, Didelot C, Houtte PV, Gerard JP, Peyron JF. NF-kB modulation and ionizing radiation: mechanisms and future directions for cancer treatment. Cancer Lett. 2006;231(2):158-68. PMID: 16399220.

    Li Q, Lu Q, Bottero V, Estepa G, Morrison L, Mercurio F and Verma IM. Enhanced NF-B activation and cellular function in macrophages lacking IB kinase 1 (IKK1). Proc Natl Acad Sci U S A. 2005;102(35):12425-30. PMID: 16116086.

    Ducut JL, Bottero V, Young DB, Shevchenko A, Mercurio F, Verma IM. ELKS, a regulatory subunit of IkB kinase complex required for NF-kB activation. Science. 2004;304(5679):1963-7. PMID: 15218148.

    Bottero V, Imbert V, Frelin C, Formento JL, Peyron JF. Monitoring NF-kB Transactivation Potential Via Real-Time PCR Quantification of IkB-a Gene Expression. Mol Diagn. 2003;7(3):187-94. PMID: 15068390.

    Tergaonkar V, Bottero V, Ikawa M, Li Q, Verma IM. IkB kinase-independent IkBa degradation pathway: functional NF-kB activity and implications for cancer therapy. Mol Cell Biol. 2003;23(22):8070-83. PMID: 14585967.

    Busuttil V, Bottero V, Frelin C, Imbert V, Ricci JE, Auberger P, Peyron JF. The blockade NF- kB activation in Jurkat leukemic T cells converts the survival agent and tumor promoter PMA into an apoptotic effector. Oncogene, 2002, vol. 21, no. 20 pp. 3213-3224. PMID: 12082637.

    Cursio R, Mari B, Louis K, Rostagno P, Saint-Paul MC, Giudicelli J, Bottero V, Anglard P, Yiotakis A, Dive V, Gugenheim J and Auberger P. Rat Liver Injury Following Normothermic 9 Ischemia is prevented by a Phosphinic Matrix Metalloproteinase Inhibitor. FASEB J. 2002;16(1):93-5. PMID: 11709491.

    Bottero V, Busuttil V, Loubat A, Magné N., Fischel JL, Milano G and Peyron JF. The topoisomerase poisons SN38 and doxorubicin activate NF-kB through the IKK complex. Cancer Research 2001, 61, 7785-7791. PMID: 11691793.

    Bottero V, Rossi F, Samson M, Mari M, Hofman P and Peyron JF. IkB-a, the NF-kB inhibitory subunit, interacts with ANT, the mitochondrial ATP/ADP translocator. J Biol Chem. 2001;276(24):21317-24. PMID: 11287411.

    Ghosh S, Tergaonkar V, Rothlin CV, Correa RG, Bottero V, Bist P, Verma IM, Hunter T. Essential role of tuberous sclerosis genes TSC1 and TSC2 in NF-kB activation and cell survival. Cancer Cell. 2006;10(3):215-26. PMID: 16959613.

    Muotri AR, Bottero V, Tergaonkar V and Correa RC. UV-Mediated NF-κB Activation is Abolished in Deficient XPC/D Primary Fibroblasts. Cell cycle, 2006;5(10):1085-9. PMID: 16721051.

    Woods NB, Bottero V, Verma IM. Therapeutic gene for XSCID gene therapy trial results in T-cell lymphoma in mice, Nature. 2006;440(7088):1123. PMID: 16641981.